Larry Daley just can't keep hold of a steady job, but he begins to think there may be hope at last as he is hired as a night watchman at the Museum of Natural History. Everything goes well on the first night for Larry. That is until everything within the Museum start to come to life. Larry now has a choice to make. Either continue on finding another job or manage everything that comes to life, which includes tiny Romans and Cowboys, a skeleton of a Tyrannosaurus Rex and Teddy Roosevelt